You can't change TreeView.HideSelection Color directly. But to change HideSelection color here is an another solution 1) TreeView.HideSelection = true 2) Declare: Public previousSeletedNode As TreeNode 'To hold reference to previously selected TreeNode 3) Private Sub TreeView1_Validating(ByVal sender As System.Object, ByVal e As System.ComponentModel.CancelEventArgs) Handles TreeView1.Validating TreeView1.SelectedNode.BackColor = Color.Yellow TreeView1.SelectedNode.ForeColor = Color.White previousSeletedNode = TreeView1.SelectedNode End Sub 4) Private Sub TreeView1_AfterSelect(ByVal sender As System.Object, ByVal e As System.Windows.Forms.TreeViewEventArgs) Handles TreeView1.AfterSelect If Not previousSeletedNode Is Nothing Then previousSeletedNode.BackColor = TreeView1.BackColor previousSeletedNode.ForeColor = TreeView1.ForeColor End If End Sub