try this:

Private Sub insertData()
Try
Dim con As SqlConnection = Nothing
Dim cmd As SqlCommand = Nothing
con = New SqlConnection(System.Configuration.ConfigurationManager.ConnectionStrings("inline").ConnectionString)
con.Open()
cmd = New SqlCommand("insert into table_name(column_Name) values ('" + TextBox1.Text + "," + TextBox2.Text + "')", con)
cmd.ExecuteNonQuery()
con.Close()

	lblMsg.Text = "Saved Successfully"
Catch ex As Exception
	lblMsg.Text = ex.Message
End Try

End Sub

Try This

<script type="text/javascript">
function jsTest()
{
if (document.getElementById("<%=txtBox1.ClientID%>").value == "") {
alert("Please Select a Pay From Option");
document.getElementById("<%=txtBox1.ClientID%>").focus();
return false;
}
else {
alert(document.getElementById("<%=txtBox1.ClientID%>").value);
}
}